Prosyn

ProSyn Linearized DNA: Efficient DNA Manufacturing with Cell-Free Technology

ProSyn Linearized DNA is a breakthrough in DNA manufacturing, utilizing cell-free technology to accelerate project timelines and reduce manpower. This innovative approach leverages enzymatic DNA synthesis through rolling circle amplification (RCA), ensuring unparalleled accuracy and a low error rate. The proprietary PowerStarryTM Primer Pool enhances the universality and stability of the DNA synthesis process.

Key Features:

• Accuracy: Unparalleled accuracy with a low error rate.
• Speed: Fast turnaround time, delivering small quantities of GMP products quickly.
• Versatility: Suitable for plasmid lengths up to 15 kb.
• Yield: High yield, with 2-3 mg of DNA produced per 10 mL of reaction.
• Purity: High purity, with approximately 95% purity as measured by IEC-HPLC.
• Scalability: Scalable from small to large quantities, with options for 10 mL, 50 mL, and 100 mL scales.

How It Works:

1. **Template Plasmid:** Single-strand template plasmid is used for the synthesis process.

2. **PowerStarryTM Primer Pool:** A proprietary primer pool that accelerates the DNA synthesis process.

3. **Rolling Circle Amplification (RCA):** Enzymatic DNA synthesis using Phi29 DNA polymerase.

4. **Endonuclease Digestion:** Final step to linearize the DNA.

Benefits:

• Efficiency: Reduces time and manpower required for DNA manufacturing.
• Flexibility: Suitable for various applications, including IND and CMC projects.
• Reliability: High-quality DNA products with consistent results.

For more information, contact GenScript ProBio at 20 Kingsbridge Rd, Piscataway, NJ 08854, USA, or visit their website at www.genscriptprobio.com.